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Our Abpromise guarantee covers the use of ab99468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 26 kDa. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21887552|
ab99468 (5μg/ml) staining of PEBP4 in paraffin embedded Human Pancreas following microwaved antigen retrieval with Tris/EDTA
buffer pH9 and HRP-staining and shows staining consistant with lysosomes.