概述

  • 产品名称Anti-PDZK1抗体
    参阅全部 PDZK1 一抗
  • 描述
    兔多克隆抗体to PDZK1
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog
  • 免疫原

    Synthetic peptide: MTSTFNPREC KLSKQEGQNY GFFLRIEKDT EGHLVRVVEK CSPAEKAGLQ , corresponding to a region within N terminal amino acids 1 - 50 of Human PDZK1 (XP_001126710)

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
  • 组织特异性Expression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas.
  • 序列相似性Belongs to the NHER family.
    Contains 4 PDZ (DHR) domains.
  • 结构域The PDZ 2 and 3 domains seem to be involved in the interaction with SLC26A3.
    Interaction with the C-terminus of CFTR could be mediated through independent binding of PDZ 1, 3 and 4 domains.
    The PDZ 1 and 3 domains seem to be involved in the interaction with SLCO1A1.
    The PDZ 1 domain interacts with BCR.
  • 细胞定位Cytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1700023D20Rik antibody
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    • AI267131 antibody
    • AI314638 antibody
    • AL022680 antibody
    • C terminal linking and modulating protein antibody
    • CAP70 antibody
    • CFTR associated protein of 70 kDa antibody
    • CFTR associated protein, 70-KD antibody
    • CFTR-associated protein of 70 kDa antibody
    • CLAMP antibody
    • D3Ertd537e antibody
    • Dietary Pi-regulated RNA-1 antibody
    • Diphor-1 antibody
    • mPDZK1 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF3 antibody
    • Na(+)/H(+) exchanger regulatory factor 3 antibody
    • Na/Pi cotransporter C-terminal-associated protein 1 antibody
    • Na/Pi cotransporter C-terminal-associated protein antibody
    • NaPi Cap1 antibody
    • NaPi-Cap1 antibody
    • NaPiCap1 antibody
    • NHERF 3 antibody
    • NHERF-3 antibody
    • NHERF3 antibody
    • NHRF3_HUMAN antibody
    • OTTHUMP00000015572 antibody
    • PDZ domain containing 1 antibody
    • PDZ domain containing protein 1 antibody
    • PDZ domain-containing protein 1 antibody
    • PDZD1 antibody
    • PDZK1 antibody
    • Sodium hydrogen exchanger regulatory factor 3 antibody
    • Sodium-hydrogen exchanger regulatory factor 3 antibody
    see all

Anti-PDZK1 antibody 图像

  • Anti-PDZK1 antibody (ab83549) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ICC/IF image of ab83549 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83549, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PDZK1 antibody (ab83549)参考文献

ab83549 has not yet been referenced specifically in any publications.

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