概述

  • 产品名称Anti-PDX1抗体
    参阅全部 PDX1 一抗
  • 描述
    兔多克隆抗体to PDX1
  • 特异性This antibody detects endogenous levels of total PDX1 protein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized non phosphopeptide derived from human PDX1 around the phosphorylation site of serine 61 (Q-G-SP-P-P).

  • 阳性对照
    • Extracts from HT-29 cells treated with serum (20 %, 15 minutes).

性能

应用

Our Abpromise guarantee covers the use of ab79388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.

靶标

  • 功能Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • 组织特异性Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • 疾病相关Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 结构域The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • 翻译后修饰Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

Anti-PDX1 antibody 图像

  • All lanes : Anti-PDX1 antibody (ab79388) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from HT-29 cells with immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 31 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

Anti-PDX1 antibody (ab79388)参考文献

This product has been referenced in:
  • Wang Q  et al. The reprogrammed pancreatic progenitor-like intermediate state of hepatic cells is more susceptible to pancreatic beta cell differentiation. J Cell Sci 126:3638-48 (2013). Rat . Read more (PubMed: 23750005) »

See 1 Publication for this product

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