概述

  • 产品名称Anti-PDS5B抗体
    参阅全部 PDS5B 一抗
  • 描述
    兔多克隆抗体to PDS5B
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between C terminanl residues 1400 and 1447 of human PDS5B

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab70299 was affinity purified using an epitope specific to PDS5B immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • AI646570 antibody
    • Androgen induced proliferation inhibitor antibody
    • Androgen-induced prostate proliferative shutoff associated protein AS3 antibody
    • APRIN antibody
    • AS3 antibody
    • AW212954 antibody
    • CG008 antibody
    • FLJ23236 antibody
    • KIAA0979 antibody
    • mKIAA0979 antibody
    • PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) antibody
    • Pds5b antibody
    • RP1 267P19.1 antibody
    • Sister chromatid cohesion protein PDS5 homolog B antibody
    see all

Anti-PDS5B antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling PDS5B with ab70299 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PDS5B antibody (ab70299) at 0.04 µg/ml

    Lane 1 : Jurkat cell lysate at 50 µg
    Lane 2 : Jurkat cell lysate at 15 µg
    Lane 3 : Jurkat cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg
    Lane 6 : 293T cell lysate at 50 µg
    Lane 7 : NIH3T3 cell lysate at 50 µg


    Predicted band size : 165 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Detection: Chemiluminescence with exposure times of 10 seconds (lanes 1-5) and 3 minutes (lanes 6-7).
  • Western Blot showing ab70299 at 1µg/ml staining PDS5B in HeLa whole cell lysates immunoprecipitated using Lane 1: ab70298 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: ab70299 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: control IgG was used for immunoprecipitation. Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-PDS5B antibody (ab70299)参考文献

ab70299 has not yet been referenced specifically in any publications.

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