The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
Detection: Chemiluminescence with exposure times of 10
seconds (lanes 1-5) and 3 minutes (lanes 6-7).
Immunoprecipitation - PDS5B antibody (ab70299)
Western Blot showing ab70299 at 1µg/ml staining PDS5B in HeLa whole cell lysates immunoprecipitated using
Lane 1: ab70298 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane).
Lane 2: ab70299 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane).
Lane 3: control IgG was used for immunoprecipitation.
Detection: Chemiluminescence with exposure time of 10