Anti-PDRG1抗体(ab74500)

概述

  • 产品名称Anti-PDRG1抗体
    参阅全部 PDRG1 一抗
  • 描述
    兔多克隆抗体to PDRG1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human PDRG1

  • 阳性对照
    • Extracts from COLO cells, MCF-7 cells, Jurkat cells or HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 15 kDa.
ELISA 1/40000.

靶标

Anti-PDRG1 antibody 图像

  • All lanes : Anti-PDRG1 antibody (ab74500) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from MCF-7 cells
    Lane 3 : extracts from Jurkat cells
    Lane 4 : extracts from HepG2 cells
    Lane 5 : extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,60 kDa. We are unsure as to the identity of these extra bands.

Anti-PDRG1 antibody (ab74500)参考文献

ab74500 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (15%)
Sample Human Cell lysate - whole cell (RKO BRAFV600E mut (colorectal cancer cell line))
Specification RKO BRAFV600E mut (colorectal cancer cell line)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Miss. Rebekka Stark

Verified customer

提交于 Oct 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"