Synthetic peptide derived from an internal sequence of human PDRG1
Extracts from COLO cells, MCF-7 cells, Jurkat cells or HepG2 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 15 kDa.
Anti-PDRG1 antibody 图像
Western blot - PDRG1 antibody (ab74500)
All lanes :
Anti-PDRG1 antibody (ab74500) at 1/500 dilution
Lane 1 :
extracts from COLO cells
Lane 2 :
extracts from MCF-7
Lane 3 :
extracts from Jurkat cells
Lane 4 :
extracts from HepG2 cells
Lane 5 :
extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size :
Observed band size :
19 kDa (
why is the actual band size different from the predicted?
Additional bands at :
40 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"