Anti-PDPK1 (phospho Y9)抗体(ab111863)

概述

  • 产品名称Anti-PDPK1 (phospho Y9)抗体
    参阅全部 PDPK1 一抗
  • 描述
    兔多克隆抗体to PDPK1 (phospho Y9)
  • 特异性ab111863 detects endogenous levels of PDPK1 only when phosphorylated at tyrosine 9.
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human PDPK1 around the phosphorylation site of tyrosine 9 (Q-L-YP-D-A).

  • 阳性对照
    • Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • 组织特异性Appears to be expressed ubiquitously.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • 细胞定位Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Anti-PDPK1 (phospho Y9) antibody 图像

  • ab111863 at 1/50 dilution staining PDPK1 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-PDPK1 (phospho Y9) antibody (ab111863)参考文献

ab111863 has not yet been referenced specifically in any publications.

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