Anti-PDPK1抗体(ab45374)

概述

  • 产品名称Anti-PDPK1抗体
    参阅全部 PDPK1 一抗
  • 描述
    兔多克隆抗体to PDPK1
  • 特异性ab45374 detects a 65 kDa protein corresponding to the apparent molecular mass of PDPK1 on SDS-PAGE immunoblots of human A431 cells and mouse brain.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    RTTSQLYDAVPIQS

    , corresponding to N terminal amino acids 3-16 of Human PDPK1

  • 阳性对照
    • Human A431 cells and mouse brain.

性能

应用

Our Abpromise guarantee covers the use of ab45374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa).
ELISA 1/2000.

靶标

  • 功能Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • 组织特异性Appears to be expressed ubiquitously.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • 细胞定位Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Anti-PDPK1 antibody (ab45374)参考文献

ab45374 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for onevial of ab31406.

To check the status of the order please contact ou...

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Thank you for your enquiry. Antibodies ab45374 and ab45375 were made from the N-terminus of human PDK-1 (accession #O15530). This region is not found in the drosophila PDK sequences found in the NCBI database, but if they would like do your own BLAS...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"