The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Western Blot: use at 1/750 to detect a 60 kD band.
(previously used at this dilution to detect PDK 1 in lysates derived from Sf9 insect cells infected with virus expressing myc-tagged human PDK 1.
Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation.
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Appears to be expressed ubiquitously.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. Contains 1 PH domain. Contains 1 protein kinase domain.
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
3 phosphoinositide dependent protein kinase 1 antibody
3-phosphoinositide-dependent protein kinase 1 antibody
hPDK 1 antibody
PkB kinase antibody
PkB kinase like gene 1 antibody
PkB like 1 antibody
Protein kinase antibody
Western blot - PDPK1 antibody (ab2495)
Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDPK1 detects 60 kDa human PDPK1 in lysates from myc-tagged human PDPK1 virus infected Sf9 cells. A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.