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Our Abpromise guarantee covers the use of ab2495 in the following tested applications.
Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDK 1 detects 60 kDa human PDK-1 in lysates from myc-tagged human PDK-1 virus infected Sf9 cells. A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.
ab2495 has not yet been referenced specifically in any publications.