概述

  • 产品名称
  • 描述
    兔多克隆抗体to PDLIM7
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 125 and 175 of Human PDLIM7 (NP_005442.2)

  • 阳性对照
    • Hela cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway.
  • 组织特异性
    Isoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver.
  • 序列相似性
    Contains 3 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
  • 结构域
    The LIM zinc-binding 2 (LIM 2) domain interacts with TBX4.
    The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5.
    Anchored to cell periphery via its N-terminal PDZ domain.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110003B01Rik antibody
    • Enigma antibody
    • LIM domain protein antibody
    • LIM domain protein enigma antibody
    • LIM mineralization protein 1 antibody
    • Lim mineralization protein 3 antibody
    • LIM mineralization protein antibody
    • LMP 1 antibody
    • LMP antibody
    • LMP1 antibody
    • LMP3 antibody
    • PDLI7_HUMAN antibody
    • PDLIM 7 antibody
    • Pdlim7 antibody
    • PDZ and LIM doamin protein 7 antibody
    • PDZ and LIM domain 7 antibody
    • PDZ and LIM domain protein 7 antibody
    • Protein enigma antibody
    see all

图片

  • All lanes : Anti-PDLIM7 antibody (ab86065) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa


    Exposure time : 30 seconds
  • Detection of PDLIM7 by Western blot of Immunprecipitate.
    ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 seconds.

文献

This product has been referenced in:
  • Ajeian JN  et al. Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl 10:51-7 (2016). Read more (PubMed: 26147903) »

See 1 Publication for this product

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