Anti-PDLIM1抗体(ab17022)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to PDLIM1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    TPPEGYEVVTVFPK

    , corresponding to amino acids 316-329 of Human PDLIM1.

  • 阳性对照
    • Human lung and heart lysates.

性能

应用

Our Abpromise guarantee covers the use of ab17022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa).

靶标

  • 功能
    Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
  • 组织特异性
    Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
  • 序列相似性
    Contains 1 LIM zinc-binding domain.
    Contains 1 PDZ (DHR) domain.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C terminal LIM domain protein 1 antibody
    • C-terminal LIM domain protein 1 antibody
    • Carboxy terminal LIM domain protein 1 antibody
    • CLIM 1 antibody
    • CLIM1 antibody
    • CLP 36 antibody
    • CLP36 antibody
    • Elfin antibody
    • Enigma antibody
    • Enigma homolog antibody
    • hCLIM 1 antibody
    • hCLIM1 antibody
    • LIM domain protein CLP 36 antibody
    • LIM domain protein CLP-36 antibody
    • LIM domain protein CLP36 antibody
    • PDLI1_HUMAN antibody
    • PDLIM 1 antibody
    • Pdlim1 antibody
    • PDZ and LIM domain 1 antibody
    • PDZ and LIM domain protein 1 antibody
    see all

Anti-PDLIM1 antibody 图像

  • Anti-PDLIM1 antibody (ab17022) at 0.1 µg/ml + Human lung lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Primary incubated for 1 hour.

实验方案

Anti-PDLIM1 antibody (ab17022)参考文献

ab17022 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Myotubes)
Loading amount
40 µg
Specification
Mouse Myotubes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ilenia Bertipaglia

Verified customer

提交于 Jun 19 2006

Thank you for your email and I'm sorry to hear that you are experiencing some difficulty with these antibodies. All 3 of these antibodies have been successfully tested for application in Western blotting. However, these antibodies have not yet been...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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