Anti-PDK4抗体[1C2BG5] (ab110336)


  • 产品名称
    参阅全部 PDK4 一抗
  • 描述
    小鼠单克隆抗体[1C2BG5] to PDK4
  • 宿主
  • 特异性
    This antibody does not cross-react with other isoforms of Human PDK (i.e. PDK1, 2 or 3).
  • 经测试应用
    适用于: Flow Cyt, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human PDK4 protein

  • 阳性对照
    • HepG2 whole cell lysate and Human heart tissue. IF/ICC: HepG2 cell line.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab110336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.
ICC/IF Use a concentration of 10 µg/ml.


  • 功能
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 组织特异性
    Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • 序列相似性
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
    • FLJ40832 antibody
    • mitochondrial antibody
    • Pdk4 antibody
    • PDK4_HUMAN antibody
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial antibody
    • Pyruvate dehydrogenase kinase 4 antibody
    • Pyruvate dehydrogenase kinase isoenzyme 4 antibody
    • Pyruvate dehydrogenase kinase isoform 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial antibody
    see all


  • ICC/IF image of ab110336 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PDK4 antibody [1C2BG5] (ab110336) at 1 µg/ml

    Lane 1 : HepG2 whole cell lysate at 10 µg
    Lane 2 : Human heart tissue at 10 µg
    Lane 3 : Recombinant Human PDK1 at 0.01 µg
    Lane 4 : Recombinant Human PDK2 at 0.01 µg
    Lane 5 : Recombinant Human PDK3 at 0.01 µg
    Lane 6 : Recombinant Human PDK4 at 0.01 µg

    Predicted band size: 46 kDa

  • Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Schumann T  et al. Deregulation of PPARß/d target genes in tumor-associated macrophages by fatty acid ligands in the ovarian cancer microenvironment. Oncotarget 6:13416-33 (2015). WB . Read more (PubMed: 25968567) »
  • Huang JV  et al. Impaired Contractile Recovery after Low-Flow Myocardial Ischemia in a Porcine Model of Metabolic Syndrome. Am J Physiol Heart Circ Physiol : (2013). WB ; Pig . Read more (PubMed: 23335793) »

See all 2 Publications for this product


Western blot
Sheep Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
50 µg
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Alex Pendleton

Verified customer

提交于 Jan 03 2018

Thank you for your phone call.

I'm sorry to hear that the antibody ab38242 is not working as expected.
I'd be happy to send you the free of charge replacement as discussed over the phone.
Please let me know which rabbit monoclonal anti...

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