概述

  • 产品名称Anti-PDK4抗体[1C2BG5]
    参阅全部 PDK4 一抗
  • 描述
    小鼠单克隆抗体[1C2BG5] to PDK4
  • 特异性This antibody does not cross-react with other isoforms of Human PDK (i.e. PDK1, 2 or 3).
  • 经测试应用适用于: Flow Cyt, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human PDK4 protein

  • 阳性对照
    • HepG2 whole cell lysate and Human heart tissue. IF/ICC: HepG2 cell line.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • 组织特异性Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • 序列相似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
    • FLJ40832 antibody
    • mitochondrial antibody
    • Pdk4 antibody
    • PDK4_HUMAN antibody
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial antibody
    • Pyruvate dehydrogenase kinase 4 antibody
    • Pyruvate dehydrogenase kinase isoenzyme 4 antibody
    • Pyruvate dehydrogenase kinase isoform 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial antibody
    see all

Anti-PDK4 antibody [1C2BG5] 图像

  • ICC/IF image of ab110336 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PDK4 antibody [1C2BG5] (ab110336) at 1 µg/ml

    Lane 1 : HepG2 whole cell lysate at 10 µg
    Lane 2 : Human heart tissue at 10 µg
    Lane 3 : Recombinant Human PDK1 at 0.01 µg
    Lane 4 : Recombinant Human PDK2 at 0.01 µg
    Lane 5 : Recombinant Human PDK3 at 0.01 µg
    Lane 6 : Recombinant Human PDK4 at 0.01 µg


    Predicted band size : 46 kDa
  • Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-PDK4 antibody [1C2BG5] (ab110336)参考文献

This product has been referenced in:
  • Huang JV  et al. Impaired Contractile Recovery after Low-Flow Myocardial Ischemia in a Porcine Model of Metabolic Syndrome. Am J Physiol Heart Circ Physiol : (2013). WB ; Pig . Read more (PubMed: 23335793) »

See 1 Publication for this product

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