RabMAb

Anti-PDK2抗体[EP1947Y] (ab76152)

概述

  • 产品名称Anti-PDK2抗体[EP1947Y]
    参阅全部 PDK2 一抗
  • 描述
    兔单克隆抗体[EP1947Y] to PDK2
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminus of human PDK2.

  • 阳性对照
    • SH-SY-5Y cell lysate; human heart muscle tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

应用

Our Abpromise guarantee covers the use of ab76152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/50.
ICC 1/50.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
    • 组织特异性Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
    • 序列相似性Belongs to the PDK/BCKDK protein kinase family.
      Contains 1 histidine kinase domain.
    • 细胞定位Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody
      • mitochondrial antibody
      • PDHK2 antibody
      • PDK2 antibody
      • PDK2_HUMAN antibody
      • Pyruvate dehydrogenase kinase isoform 2 antibody
      • Pyruvate dehydrogenase kinase, isozyme 2 antibody
      • Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
      see all

    Anti-PDK2 antibody [EP1947Y] 图像

    • ab76152 at 1/50 dilution staining PDK2 in human heart muscle by Immunohistochemistry, Paraffin-embedded tissue.
    • Anti-PDK2 antibody [EP1947Y] (ab76152) at 1/1000 dilution + SH-SY-5Y cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution
      Developed using the ECL technique

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Overlay histogram showing HepG2 cells stained with ab76152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76152, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-PDK2 antibody [EP1947Y] (ab76152)参考文献

    ab76152 has not yet been referenced specifically in any publications.

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