Anti-PDK2抗体[3F2D7] (ab201964)
Key features and details
- Mouse monoclonal [3F2D7] to PDK2
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Rat, Human, African green monkey
- Isotype: IgG2b
概述
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产品名称
Anti-PDK2抗体[3F2D7]
参阅全部 PDK2 一抗 -
描述
小鼠单克隆抗体[3F2D7] to PDK2 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Rat, Human, African green monkey
预测可用于: Mouse -
免疫原
Recombinant fragment corresponding to Human PDK2 aa 150 to the C-terminus. Expressed in E. Coli.
Database link: Q15119 -
阳性对照
- A431 cells; Human recombinant PDK2 (aa178-404) protein; Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate; Jurkat , C6), COS7, K562 and A431 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
3F2D7 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201964于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 46 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 46 kDa. |
Flow Cyt
1/200 - 1/400. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
组织特异性
Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung. -
序列相似性
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5164 Human
- Entrez Gene: 18604 Mouse
- Entrez Gene: 81530 Rat
- Omim: 602525 Human
- SwissProt: Q15119 Human
- SwissProt: Q9JK42 Mouse
- SwissProt: Q64536 Rat
- Unigene: 256667 Human
see all -
别名
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody
- mitochondrial antibody
- PDHK2 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: PDK2 knockout HAP1 cell lysate (40 µg)
Lane 3: SHSY5Y cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab201964 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.ab201964 was shown to recognize PDK2 when PDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and PDK2 knockout samples were subjected to SDS-PAGE. Ab201964 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) ab216772 and IRDye® 680 Goat anti-Rabbit IgG (H + L) ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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Flow cytometric analysis of A431 cells labeling PDK2 with ab201964 at 1/200 dilution (green). Negative control (red).
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All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : C6 cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : A431 cell lysate
Predicted band size: 46 kDa -
All lanes : Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 46 kDa -
Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution + Human recombinant PDK2 (aa178-404) protein
Predicted band size: 46 kDa
数据表及文件
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Datasheet download
文献 (0)
ab201964 尚未被引用在任何文献中。