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Full length protein.
Our Abpromise guarantee covers the use of ab3672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/50 - 1/100.|
|WB||1/1000. Detects a band of approximately 60 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/600. PubMed: 19059915|
ab3672 at 1/400 staining mouse kidney tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was incubated with the primary antibody for 30 minutes at 22°C and then an HRP conjugated goat anti-rabbit antibody was used as the secondary.
Chinese Hamster Ovary cells stained for PDI using ab3672 at 1/200 dilution ICC/IF. Cy3®-labeled secondary antibody.
ab3672 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr. Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours at 25°C. A TRITC conjugated pig anti-rabbit IgG antibody, diluted 1/1000, was used as the secondary.
ab3672 staining PDI - ER in human HaCaT Keratinocyte by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.25% triton X-100 buffer and blocked in 2.5% BSA plus 1% goat serum for 1 hour at 4°C. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.
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