Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids 290-318 of Human PDHA2 (NP_005381.1)
NCI-H460 cell line lysates and human testis tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
Purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 43 kDa.
1/50 - 1/100.
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Testis. Expressed in postmeiotic spermatogenic cells.
Information by UniProt
EC 18.104.22.168 antibody
Western blot - Anti-PDHA2 antibody (ab107015)
Anti-PDHA2 antibody (ab107015) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA2 antibody (ab107015)
Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using ab107015 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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