Anti-PDGFC抗体(ab93899)

概述

  • 产品名称Anti-PDGFC抗体
    参阅全部 PDGFC 一抗
  • 描述
    兔多克隆抗体to PDGFC
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PDGFC.

  • 阳性对照
    • This antibody gave a positive signal in Human platelet whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab93899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa).

靶标

  • 功能Potent mitogen and chemoattractant for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. Appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. Acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding to receptors induces their activation by tyrosine phosphorylation. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintainance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Seems to be involved in palatogenesis.
  • 组织特异性Expressed in the fallopian tube, vascular smooth muscle cells in kidney, breast and colon and in visceral smooth muscle of the gastrointestinal tract. Highly expressed in retinal pigment epithelia. Expressed in medulloblastoma. In the kidney, constitutively expressed in parietal epithelial cells of Bowman's capsule, tubular epithelial cells and in arterial endothelial cells (at protein level). Highly expressed in the platelets, prostate, testis and uterus. Higher expression is observed in uterine leiomyomata. Weaker expression in the spleen, thymus, heart, pancreas, liver, ovary cells and small intestine, and negligible expression in the colon and peripheral blood leukocytes.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
    Contains 1 CUB domain.
  • 发展阶段In the fetal kidney, detected in the developing mesangium, ureteric bud epithelium and the undifferentiated mesenchyme (at protein level).
  • 翻译后修饰Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after basic residues in the hinge region (region connecting the CUB and growth factor domains) gives rise to the receptor-binding form. Cleaved by PLAT and PLG.
    Sumoylated by SUMO1.
    N-glycosylated.
  • 细胞定位Cytoplasm. Secreted. Nucleus. Cytoplasmic granule. Sumoylated form is predominant in the nucleus. Stored in alpha granules in platelets. Membrane associated when bound to receptors.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Fallotein antibody
    • hSCDGF antibody
    • PDGF-C antibody
    • PDGFC antibody
    • PDGFC latent form antibody
    • PDGFC receptor-binding form antibody
    • PDGFC_HUMAN antibody
    • Platelet derived growth factor C antibody
    • Platelet-derived growth factor C, receptor-binding form antibody
    • SCDGF antibody
    • Secretory growth factor like protein antibody
    • Secretory growth factor like protein fallotein antibody
    • Spinal cord derived growth factor antibody
    • Spinal cord-derived growth factor antibody
    • VEGF E antibody
    • VEGF-E antibody
    see all

Anti-PDGFC antibody 图像

  • Anti-PDGFC antibody (ab93899) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab93899 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93899 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PDGFC antibody (ab93899)参考文献

ab93899 has not yet been referenced specifically in any publications.

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As discussed on the telephone, I am sorry to confirm that as far as we are aware, ab93899 and ab122915have never been tested with samples from mouse. All tested species cross-reactivity informatio...

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