RabMAb

Anti-PDGF Receptor beta (phospho Y857)抗体[EP2136Y] (ab62367)

概述

  • 产品名称Anti-PDGF Receptor beta (phospho Y857)抗体[EP2136Y]
    参阅全部 PDGF Receptor beta 一抗
  • 描述
    兔单克隆抗体[EP2136Y] to PDGF Receptor beta (phospho Y857)
  • 特异性ab62367 detects PDGF Receptor beta phosphorylated at Tyrosine 857.
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse
  • 免疫原

    A synthetic phosphopeptide corresponding to residues surrounding Tyrosine 857 of the human PDGF Receptor beta

  • 阳性对照
    • NIH3T3 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab62367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 123 kDa).
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
    • 疾病相关Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
      Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
      Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
      Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
      Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta platelet derived growth factor receptor antibody
      • Beta-type platelet-derived growth factor receptor antibody
      • CD 140B antibody
      • CD140 antigen-like family member B antibody
      • CD140b antibody
      • CD140b antigen antibody
      • IBGC4 antibody
      • IMF1 antibody
      • JTK12 antibody
      • OTTHUMP00000160528 antibody
      • PDGF R beta antibody
      • PDGF-R-beta antibody
      • PDGFR 1 antibody
      • PDGFR antibody
      • PDGFR beta antibody
      • PDGFR1 antibody
      • PDGFRB antibody
      • PGFRB_HUMAN antibody
      • Platelet derived growth factor receptor 1 antibody
      • Platelet derived growth factor receptor beta antibody
      • Platelet derived growth factor receptor beta polypeptide antibody
      see all

    Anti-PDGF Receptor beta (phospho Y857) antibody [EP2136Y] 图像

    • All lanes : Anti-PDGF Receptor beta (phospho Y857) antibody [EP2136Y] (ab62367) at 1/1000 dilution

      Lane 1 : NIH3T3 cell lysates (untreated)
      Lane 2 : NIH3T3 cell lysates treated with PDGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit, HRP labeled, at 1/2000 dilution

      Predicted band size : 123 kDa
      Observed band size : 190 kDa (why is the actual band size different from the predicted?)

    Anti-PDGF Receptor beta (phospho Y857) antibody [EP2136Y] (ab62367)参考文献

    ab62367 has not yet been referenced specifically in any publications.

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