Anti-PDGF Receptor beta (phospho Y1021)抗体(ab62437)

概述

  • 产品名称
    Anti-PDGF Receptor beta (phospho Y1021)抗体
    参阅全部 PDGF Receptor beta 一抗
  • 描述
    兔多克隆抗体to PDGF Receptor beta (phospho Y1021)
  • 特异性
    This antibody detects endogenous levels of PDGF Receptor beta only when phosphorylated at tyrosine 1021.
  • 经测试应用
    适用于: IHC-P, ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human PDGF Receptor beta around the phosphorylation site of tyrosine 1021 (N-D-YP-I-I).

  • 阳性对照
    • Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab62437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

靶标

  • 功能
    Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • 疾病相关
    Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

图片

  • ab62437 staining PDGF Receptor beta (phospho Y1021) (green) in mouse primary embryonic epicardial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA + 10% goat serum + 0.1% Triton X-100 in PBS for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - phalloidin.

    See Abreview

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab62437 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.


  • Performed under reducing conditions.

    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 seconds

    This image is courtesy of an Abreview submitted by Lars Ruether

    All lanes : PDGF Receptor beta (phospho Y1021) antibody (ab62437) at 1/100 dilution (in TBST for 12 hours at 4°C) + 100µg whole cell lysate of Human glioblastoma (A172 cells)(unstimulated Lane 1 or stimulated Lanes 2-7)

    Secondary: A Biotin-conjugated Goat polyclonal at 1/10000 dilution
    developed using the ECL technique

    Blocking Step: 5% BSA for 90 minutes at room temperature.

    See Abreview

  • ab62437 staining the PDGF Receptor beta (phospho Y1021) in epithelial cells in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% BSA for 20 minutes at room temperature; antigen retrieval was by heat mediation with citric acid (pH6.0). Samples were incubated with primary antibody (1/50 in 0.5% BSA/PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

文献

ab62437 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 12 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (primary embryonic epicardial)
Specification
primary embryonic epicardial
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 13 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue (Brian section)
Specification
Brian section
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 18 2013

Thank you for your message.

I would like to reassure you that although these antibodies are recommended to be stored at -20oC, I would not expect being at 4oC for a few days to significantly affect their performance. However, I would recomme...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human non-neoplastic cortex)
Specification
human non-neoplastic cortex
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrit acid pH 6.1
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (epithelia cells in mouse brain tissue)
Specification
epithelia cells in mouse brain tissue
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Brain (Glioblastoma))
Loading amount
100 µg
Specification
Brain (Glioblastoma)
Treatment
PDGF-BB: 20 & 40 ng/ml for 5, 10, 15 min.
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Lars Rüther

Verified customer

提交于 Sep 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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