Anti-PDGF Receptor alpha (phospho Y849)抗体(ab79318)

概述

  • 产品名称
    Anti-PDGF Receptor alpha (phospho Y849)抗体
    参阅全部 PDGF Receptor alpha 一抗
  • 描述
    兔多克隆抗体to PDGF Receptor alpha (phospho Y849)
  • 特异性
    ab79318 detects endogenous levels of PDGF Receptor alpha only when phosphorylated at tyrosine 849.
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of tyrosine 849 (S-N-YP-V-S).

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab79318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. 1/50 (see Abreview)
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 123 kDa).
ELISA 1/5000.

靶标

  • 功能
    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 组织特异性
    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 疾病相关
    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

图片

  • All lanes : Anti-PDGF Receptor alpha (phospho Y849) antibody (ab79318) at 1/500 dilution

    Lane 1 : 293 cells extract
    Lane 2 : 293 cells extract with Immunizing phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 123 kDa
    Observed band size : 133 kDa (why is the actual band size different from the predicted?)
  • ab79318 staining the PDGF Receptor alpha (phospho Y849) in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% BSA for 20 minutes at room temperature; antigen retrieval was by heat mediation with citric acid (pH6.0). Samples were incubated with primary antibody (1/50 in 0.5% BSA/PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

文献

ab79318 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Formaldehyde
Permeabilization
Yes - Tritonx100 0.1% in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (glioblastoma multiforme brain section)
Specification
glioblastoma multiforme brain section
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 16 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (Brain Glioblastoma)
Loading amount
120 µg
Specification
Brain Glioblastoma
Treatment
PDGF 20/40 ng/ml 5, 10, 15 min
Gel Running Conditions
Reduced Denaturing (7,5 %)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Oct 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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