Anti-PDGF Receptor alpha抗体(ab71008)

概述

性能

应用

Our Abpromise guarantee covers the use of ab71008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 功能Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 组织特异性Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 疾病相关Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Anti-PDGF Receptor alpha antibody 图像

  • All lanes : Anti-PDGF Receptor alpha antibody (ab71008) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the PDGF Receptor alpha gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 123 kDa
    Observed band size : 123 kDa
    Additional bands at : 100 kDa,32 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ab71008, at 1/10 dilution, staining PDGF Receptor alpha in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PDGF Receptor alpha antibody (ab71008)参考文献

ab71008 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (glioblastoma multiforme brain section)
Specification glioblastoma multiforme brain section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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提交于 Nov 16 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 16 2010

Application Western blot
Sample Human Cell lysate - whole cell (Brain)
Loading amount 100 µg
Specification Brain
Treatment PDGF 20-40 ng/ml 5min, 10 min, 15 min
Gel Running Conditions Reduced Denaturing (7,5 %)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

提交于 Oct 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"