Anti-PDGF B抗体[MM0014-5F66] (ab51869)

概述

  • 产品名称Anti-PDGF B抗体[MM0014-5F66]
    参阅全部 PDGF B 一抗
  • 描述
    小鼠单克隆抗体[MM0014-5F66] to PDGF B
  • 经测试应用适用于: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human)

性能

应用

Our Abpromise guarantee covers the use of ab51869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24512807
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP 1/50.
IHC-P 1/50 - 1/200.
WB 1/100 - 1/200. Predicted molecular weight: 27 kDa.

靶标

  • Information by UniProt
  • 数据库链接
  • 别名
    • Becaplermin antibody
    • PDGF 2 antibody
    • PDGF B chain antibody
    • PDGF subunit B antibody
    • PDGF-2 antibody
    • PDGF2 antibody
    • Pdgfb antibody
    • PDGFB_HUMAN antibody
    • Platelet derived growth factor 2 antibody
    • Platelet-derived growth factor B chain antibody
    • Platelet-derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor subunit B antibody
    • Proto-oncogene c-Sis antibody
    • SIS antibody
    • SSV antibody
    see all

Anti-PDGF B antibody [MM0014-5F66] 图像

  • ab51869 at 1/200 dilution, staining PDGF BB in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Overlay histogram showing SHSY-5Y cells stained with ab51869 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51869, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PDGF B antibody [MM0014-5F66] (ab51869)参考文献

This product has been referenced in:
  • Miyata T  et al. The roles of platelet-derived growth factors and their receptors in brain radiation necrosis. Radiat Oncol 9:51 (2014). IHC-P, ICC/IF ; Human . Read more (PubMed: 24512807) »
  • Niu F  et al. Tat 101-mediated enhancement of brain pericyte migration involves platelet-derived growth factor subunit B homodimer: implications for human immunodeficiency virus-associated neurocognitive disorders. J Neurosci 34:11812-25 (2014). IHC-P, ICC/IF . Read more (PubMed: 25164676) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (3T3)
Total protein in input 5e+007 cells
Specification 3T3
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

提交于 Feb 05 2013

Thank you for contacting us, I apologize for the delay in my reply. I would recommend ab114130 for your purposes. The immunogen for this antibody shows no significant similarity to PDGFA, and it is slightly less likely to react with mouse than our othe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"