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Our Abpromise guarantee covers the use of ab16829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.|
|IHC-P||Use at an assay dependent concentration.|
ab16829 staining PDGF BB (green) in mouse adipose tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was enzymatic in Tris (pH 10). Samples were incubated with primary antibody (1/100 in anitbody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Dlue - DAPI, Red - Elastin.