Anti-PDGF A抗体(ab38562)

概述

  • 产品名称Anti-PDGF A抗体
  • 描述
    兔多克隆抗体to PDGF A
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human PDGF A (N terminal) conjugated to keyhole limpet haemocyanin.

  • 阳性对照
    • HL60 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab38562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Anti-PDGF A antibody 图像

  • Anti-PDGF A antibody (ab38562) at 1/100 dilution + HL60 cell lysate

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 36 kDa,40 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Anti-PDGF A antibody (ab38562)参考文献

ab38562 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I have checked the image which is not very nice. The black dots explains, the buffers should be filtered. Could you try filtering all the buffers?
- Wash the primary and secondary antibody with TBST for 10 minutes 1 ti...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your email.

The explanation of different band sizes was for PDGF AA protein. It is the protein that customer is interested in, so I am sorry the experiments has to be determined by end user. We unfortunately cannot do experiment ...

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Thank you for your email.

The PDGF-AA is a homodimer of PDGF-A protein (http://www.uniprot.org/uniprot/P04085). The PDGF-A protein further gets cleaved, amino acid 87-211. Due to these post translational modifications the observed band size w...

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Thanks you for your email. I am sorry for contacting you late because I was in touch with lab. My colleague has confirmed that they have observed a band of 24kDa with HL60 cell line lysates when characterized this ab. So I am sorry I am unable to find ...

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Thank you for your enquiry regarding ab38562 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"