概述

  • 产品名称Anti-PDE8B抗体
    参阅全部 PDE8B 一抗
  • 描述
    兔多克隆抗体to PDE8B
  • 特异性This antibody has no cross-reactivity against PDE8A protein or any other PDE family members.
  • 经测试应用适用于: WB, IP, IHC-Fr, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human).

  • 阳性对照
    • Rat testis and heart.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液'Stabilization buffer' with 0.02% Sodium Azide.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 98-100 kDa.
IP 1/250.
IHC-Fr Use at an assay dependent concentration. PubMed: 18431404
Functional Studies Use at an assay dependent concentration. This antibody is ideal for activity measurement and screening of various PDE8B enzyme variants in sample.

靶标

  • 功能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.
  • 组织特异性Abundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates.
  • 通路Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 疾病相关Defects in PDE8B are the cause of striatal degeneration autosomal dominant (ADSD) [MIM:609161]. It is a movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present.
  • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3' 5' cyclic nucleotide phosphodiesterase 8B antibody
    • 3'5' cyclic nucleotide phosphodiesterase 8B antibody
    • Cell proliferation-inducing gene 22 protein antibody
    • FLJ11212 antibody
    • High affinity cAMP specific and IBMX insensitive 3' 5' cyclic phosphodiesterase 8B antibody
    • High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8B antibody
    • High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B antibody
    • HSPDE 8B antibody
    • HsPDE8B antibody
    • PDE 8B antibody
    • PDE8B antibody
    • PDE8B_HUMAN antibody
    • Phosphodiesterase 8B antibody
    • Phosphodiesterase8B antibody
    • PIG22 antibody
    see all

Anti-PDE8B antibody 图像

  • Western Blot analysis of PDE8B with ab14621 diluted 1:500. Lane 1 - rat testis; lane 2 - rat heart.

Anti-PDE8B antibody (ab14621)参考文献

This product has been referenced in:
  • Wilmot Roussel H  et al. Identification of gene expression profiles associated with cortisol secretion in adrenocortical adenomas. J Clin Endocrinol Metab 98:E1109-21 (2013). Read more (PubMed: 23539725) »
  • Horvath A  et al. A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex. Eur J Hum Genet 16:1245-53 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18431404) »

See all 2 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (whole brain lysate homogenate)
Total protein in input 2000 µg
Specification whole brain lysate homogenate
Immuno-precipitation step Protein G
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提交于 Jul 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"