概述

  • 产品名称
  • 描述
    兔多克隆抗体to PDE8A
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region between residue 779 and 829 of Human PDE8A (NP_002596.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
  • 组织特异性
    Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
  • 通路
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 序列相似性
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域
    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5''-cyclic phosphodiesterase 8A antibody
    • cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
    • FLJ16150 antibody
    • High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody
    • High affinity cAMP-specific and IBMX-insensitive 3'' antibody
    • HsT19550 antibody
    • PDE 8A antibody
    • PDE8A antibody
    • PDE8A_HUMAN antibody
    • Phosphodiesterase 8A antibody
    • Phosphodiesterase8A antibody
    • Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody
    see all

图片

  • All lanes : Anti-PDE8A antibody (ab109597) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg


    Predicted band size : 93 kDa
  • Detection of PDE8A by Western Blot of Immunprecipitate.
    Lane 1: ab109597 at 1µg/ml staining PDE8A in HeLa whole cell lysate immunoprecipitated using ab109597 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

文献

ab109597 has not yet been referenced specifically in any publications.

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