参阅全部 PDE5A 一抗
- 特异性This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074; NP_236914; NP_246273). [NP_237223 has been retracted].
Rat, Cow, Dog
C-LINGESG QAKRN , corresponding to C terminal amino acids 864-875 of Human PDE5A.
- Human lung lysate. Lysate prepared from transfected HEK293 cells transiently expressing PDE5A.
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3.
Concentration information loading...
- 纯度Immunogen affinity purified
- 纯化说明This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
- Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with PDE5A peptide (864-875) (ab45684).
- 功能Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
- 组织特异性Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
- 通路Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
- 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains.
- 结构域Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
- 翻译后修饰Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
Information by UniProt
- 5''-cyclic phosphodiesterase antibody
- CGB PDE antibody
- CGB-PDE antibody
Anti-PDE5A antibody (ab28761)参考文献
has not yet been referenced specifically in any publications.
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