概述

  • 产品名称Anti-PDE5A抗体
    参阅全部 PDE5A 一抗
  • 描述
    山羊多克隆抗体to PDE5A
  • 特异性This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074; NP_236914; NP_246273). [NP_237223 has been retracted].
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-LINGESG QAKRN

    , corresponding to C terminal amino acids 864-875 of Human PDE5A.

  • 阳性对照
    • Human lung lysate. Lysate prepared from transfected HEK293 cells transiently expressing PDE5A.

性能

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应用

Our Abpromise guarantee covers the use of ab28761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with PDE5A peptide (864-875) (ab45684).

靶标

  • 功能Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
  • 组织特异性Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
  • 通路Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • 结构域Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
  • 翻译后修饰Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5''-cyclic phosphodiesterase antibody
    • CGB PDE antibody
    • CGB-PDE antibody
    • CGBPDE antibody
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific phosphodiesterase antibody
    • cGMP binding/cGMP specific phosphodiesterase antibody
    • cGMP specific 3'5' cyclic phosphodiesterase antibody
    • cGMP specific phosphodiesterase PDE5A2 antibody
    • cGMP specific phosphodiesterase type 5A antibody
    • cGMP-binding cGMP-specific phosphodiesterase antibody
    • cGMP-specific 3'' antibody
    • CN5A antibody
    • CN5N antibody
    • PDE 5 antibody
    • PDE 5A antibody
    • PDE5 antibody
    • Pde5a antibody
    • PDE5A_HUMAN antibody
    • PDE5A1 antibody
    • Phosphodiesterase 5A antibody
    • Phosphodiesterase 5A cGMP specific antibody
    • Phosphodiesterase isozyme 5 antibody
    see all

Anti-PDE5A antibody 图像

  • Anti-PDE5A antibody (ab28761) at 0.3 µg/ml + Human lung lysate 30µg total protein per lane

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
  • Anti-PDE5A antibody (ab28761) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing PDE5A (isoform 2)

    Predicted band size : 100 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

Anti-PDE5A antibody (ab28761)参考文献

ab28761 has not yet been referenced specifically in any publications.

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