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Our Abpromise guarantee covers the use of ab121375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500.|
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Immunocytochemistry/Immunofluorescence analysis of A-431 cells labelling PDE4DIP with ab121375 shows positivity in the Golgi apparatus.
ab121375 has not yet been referenced specifically in any publications.