概述
-
产品名称Anti-PDE4D3抗体
参阅全部 PDE4D3 一抗 -
描述山羊多克隆抗体to PDE4D3
-
宿主Goat
-
经测试应用适用于: WBmore details
-
种属反应性与反应: Rat
预测可用于: Human
-
免疫原
Synthetic peptide:
HVNNFPFRRHS-C
, corresponding to N terminal amino acids 3-12 of Human PDE4D3 (variant AAA97892.1). -
阳性对照
- Rat Brain lysate.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
-
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
-
Positive Controls
应用
Our Abpromise guarantee covers the use of ab59021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 77 kDa. |
靶标
-
相关性Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprised of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.
-
细胞定位Cell Membrane and Cytoplasmic
-
数据库链接
- Entrez Gene: 5144 Human
- Entrez Gene: 24627 Rat
- Omim: 600129 Human
- SwissProt: Q08499 Human
- SwissProt: P14270 Rat
- Unigene: 117545 Human
- Unigene: 95959 Rat
-
别名
- DPDE3 antibody
- hPDE4D3 antibody
- HSPDE4D antibody
see all
图片
-
Western blot - Anti-PDE4D3 antibody (ab59021)Anti-PDE4D3 antibody (ab59021) at 0.01 µg/ml + Rat Brain lysate (35µg protein in RIPA buffer)
Observed band size: 77 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
文献
ab59021 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"