产品名称 Anti-PDE4D抗体 参阅全部 PDE4D 一抗
WB, ICC/IF more details
Synthetic peptide corresponding to a region within amino acids 610 and 673 of Human PDE4D (NP_006194; UniProt ID: Q08499 isoform 2).
WB: HeLa S3 whole cell lysate
ICC/IF: A431 cells
存放说明 Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: for isoform 2, 76 kDa.
1/100 - 1/200.
功能 Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
组织特异性 Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
通路 Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
疾病相关 Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
序列相似性 Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
翻译后修饰 Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
细胞定位 Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
Information by UniProt
cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
5''-cyclic phosphodiesterase 4D antibody
Anti-PDE4D antibody 图像
Western blot - PDE4D antibody (ab97796)
Anti-PDE4D antibody (ab97796) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg Predicted band size : for isoform 2, 76 kDa
Immunocytochemistry/ Immunofluorescence - PDE4D antibody (ab97796)
Immunofluorescence analysis of PDE4D in paraformaldehyde fixed A431, using ab97796 at a 1/200 dilution. Lower image was merged with DNA probe.
Anti-PDE4D antibody (ab97796)参考文献
has not yet been referenced specifically in any publications.
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