Anti-PDE4D抗体(ab59249)

概述

  • 产品名称Anti-PDE4D抗体
    参阅全部 PDE4D 一抗
  • 描述
    兔多克隆抗体to PDE4D
  • 特异性This antibody detects endogenous levels of total PDE4D protein.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190/53 (RESPFL).

  • 阳性对照
    • Human colon carcinoma and K562 cell extracts treated with H2O2 Human colon carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab59249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 91 kDa).

靶标

  • 功能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 组织特异性Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • 通路Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 疾病相关Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻译后修饰Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • 细胞定位Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Anti-PDE4D antibody 图像

  • All lanes : Anti-PDE4D antibody (ab59249) at 1/500 dilution

    Lane 1 : K562 cell extracts treated with H2O2 (100uM, 30mins)
    Lane 2 : K562 cell extracts treated with H2O2 (100uM, 30mins) with immunizing peptide


    Predicted band size : 91 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab59249 at 1/50 dilution staining PDE4D in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunizing peptide.

Anti-PDE4D antibody (ab59249)参考文献

ab59249 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"