概述

  • 产品名称
    Anti-PDE4A5抗体
  • 描述
    兔多克隆抗体to PDE4A5
  • 特异性
    PDE4A5 variant specific. Does not cross-react with PDE4A8 or other PDE4A proteins including PDE4A4, PDE4A1 and PDE4Ax or with other PDE family members.
  • 经测试应用
    适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Rat)- unique N-terminal sequence PDE4A5 synthetic cyclic peptide, modified to achieve desired antigenicity and linker for covalent coupling to blue carrier protein.

  • 阳性对照
    • Rat hippocampal extracts; SHSy-5Y cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • 存储溶液
    Antibody stabilization buffer, with preservatives, consisting of Hepes, NaCl, KCl, glycerol, BSA and chaotropic agents.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/750 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 组织特异性
    Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • 通路
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 序列相似性
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻译后修饰
    Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • 细胞定位
    Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5''-cyclic phosphodiesterase 4A antibody
    • cAMP-specific 3'' antibody
    • DPDE2 antibody
    • PDE46 antibody
    • PDE4A antibody
    • PDE4A_HUMAN antibody
    • Phosphodiesterase 4 antibody
    • Phosphodiesterase 4A antibody
    see all

Anti-PDE4A5 antibody 图像

  • Anti-PDE4A5 antibody (ab42094) + PDE4A5

    Predicted band size : 108 kDa
    Observed band size : 108 kDa

Anti-PDE4A5 antibody (ab42094)参考文献

This product has been referenced in:
  • Wilson NM  et al. Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. Front Syst Neurosci 10:5 (2016). WB . Read more (PubMed: 26903822) »
  • Oliva AA  et al. Phosphodiesterase isoform-specific expression induced by traumatic brain injury. J Neurochem 123:1019-29 (2012). Read more (PubMed: 23057870) »

See all 2 Publications for this product

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