The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/750 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Use at an assay dependent concentration.
功能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
组织特异性Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
翻译后修饰Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA. Proteolytically cleaved by caspase-3.
细胞定位Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.