概述

  • 产品名称Anti-PDE4A抗体
    参阅全部 PDE4A 一抗
  • 描述
    小鼠多克隆抗体to PDE4A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human protein (isoform 4: NP_006193.1)

  • 阳性对照
    • 293T cells transfected with PDE4A

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Predicted molecular weight: 98 kDa (isoform 1) .


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
    • 组织特异性Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
    • 通路Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
    • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
    • 翻译后修饰Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
      Proteolytically cleaved by caspase-3.
    • 细胞定位Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5''-cyclic phosphodiesterase 4A antibody
      • cAMP specific phosphodiesterase antibody
      • cAMP-specific 3'' antibody
      • cAMP-specific 3',5'-cyclic phosphodiesterase 4A antibody
      • DPDE2 antibody
      • dunce like phosphodiesterase E2 antibody
      • PDE 4A antibody
      • PDE4 antibody
      • PDE46 antibody
      • PDE4A antibody
      • PDE4A_HUMAN antibody
      • PDE4A11 antibody
      • Phosphodiesterase 4A antibody
      • phosphodiesterase 4A cAMP specific antibody
      • Phosphodiesterase 4A, cAMP-specific (dunce antibody
      • phosphodiesterase E2 dunce homolog antibody
      • Phosphodiesterase E2 dunce homolog, Drosophila antibody
      • phosphodiesterase isozyme 4 antibody
      • phosphodiesterase type 4A11 antibody
      see all

    Anti-PDE4A antibody 图像

    • All lanes : Anti-PDE4A antibody (ab76853) at 1/500 dilution

      Lane 1 : PDE4A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 98 (isoform 1) kDa
      Observed band size : 74 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Anti-PDE4A antibody (ab76853)参考文献

    ab76853 has not yet been referenced specifically in any publications.

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