概述

  • 产品名称
  • 描述
    兔多克隆抗体to PDE4A
  • 特异性
    This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), A5 (109 kDa), A8 (106 kDa), Ax (102 kDa) and a 76 kDa testis specific A variant.
  • 经测试应用
    适用于: ICC/IF, WB, IP, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Synthetic peptide corresponding to the C-terminal region of PDE4A (common to all PDE4A proteins).

  • 阳性对照
    • recombinant protein (PDE4A1, PDE4A5, PDE4A8, PDE4A10, PDE4A11)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 98 kDa.Can be blocked with PDE4A peptide (ab169988). Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant).
IP 1/250.
ICC 1/250.
IHC-P Use at an assay dependent concentration. PubMed: 19924104

靶标

  • 功能
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 组织特异性
    Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • 通路
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 序列相似性
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻译后修饰
    Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • 细胞定位
    Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5''-cyclic phosphodiesterase 4A antibody
    • cAMP specific phosphodiesterase antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A antibody
    • DPDE2 antibody
    • dunce like phosphodiesterase E2 antibody
    • PDE 4A antibody
    • PDE4 antibody
    • PDE46 antibody
    • PDE4A antibody
    • PDE4A_HUMAN antibody
    • PDE4A11 antibody
    • Phosphodiesterase 4A antibody
    • phosphodiesterase 4A cAMP specific antibody
    • Phosphodiesterase 4A, cAMP-specific (dunce antibody
    • phosphodiesterase E2 dunce homolog antibody
    • Phosphodiesterase E2 dunce homolog, Drosophila antibody
    • phosphodiesterase isozyme 4 antibody
    • phosphodiesterase type 4A11 antibody
    see all

文献

This product has been referenced in:
  • Cheng H  et al. siRNA-mediated silencing of phosphodiesterase 4B expression affects the production of cytokines in endotoxin-stimulated primary cultured microglia. Exp Ther Med 12:2257-2264 (2016). WB ; Rat . Read more (PubMed: 27698721) »
  • Ji Q  et al. Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation. Mol Med Rep 13:1914-22 (2016). Read more (PubMed: 26706904) »

See all 14 Publications for this product

客户评价及客户问答

Thank you for your enquiry. 1) PDE4A antibody (ab14607) does not cross react to any known PDE4 subtype except all the PDE4A subtype variants including PDE4A1, A5, Ax, A8, it has little or no reactivity towards PDE4A10 and PDE4A11. We have tested fo...

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The buffer contains mainly Tris/glycine buffer pH 7.4-7.8; cryo-protective agents, stabilizing proteins (BSA) and glycerol. There is also sodium azide as preservative. The exact composition of this buffer is proprietary.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements for ab14611 and ab14613with the order number 1137676.

To check the status...

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Thank you for contacting us.

I have been in contact with the lab about this product, the epitope that this product recognizes is not mapped, so we don't know if it would recognize all the isoforms of PDE4A.
I would also like to inform you...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat fibroblasts)
Loading amount
5 µg
Specification
Rat fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Abcam user community

Verified customer

提交于 Sep 19 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (vomeronasal organ VNO)
Specification
vomeronasal organ VNO
Fixative
Paraformaldehyde
Blocking step
gelatine plus serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

提交于 Oct 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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