概述

  • 产品名称Anti-PDE4抗体
  • 描述
    兔多克隆抗体to PDE4
  • 特异性This antibody detects all known PDE4 A and D variants including PDE4A1, A5, Ax, A8 and PDE4D1-D5. It has low affinity for PDE4B variants.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • 阳性对照
    • Rat brain.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Thimerosal / Merthiolate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 相关性The cAMP-specific Phosphodiesterase type 4 (PDE4) family is comprised of 4 genes (PDE4 A, B, C and D) with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The cAMP-dependent protein kinase A modulates the enzyme activity of some PDE4 family members by phosphorylation / dephosphorylation mechanisms. Such short-term regulatory mechanisms are necessary for rapid changes in the cAMP levels in cells. PDE4 family selective inhibitor, rolipram inhibits all members of the PDE4 family. In rodents inhibition of PDE4 enzymes by rolipram attenuated short- and long-term memory impairment produced by the administration of scopolamine and MK801. Interaction of these enzymes with cellular scaffolding proteins plays important role in PDE4 compartmentalization and cAMP-dependent signaling.
  • 细胞定位Cell Membrane
  • 数据库链接
  • 别名
    • PDE4A antibody
    • PDE4B antibody
    • PDE4C antibody
    • PDE4D antibody
    • Phosphodiesterase 4 antibody
    • Phosphodiesterase 4A antibody
    • Phosphodiesterase 4B antibody
    • Phosphodiesterase 4C antibody
    • Phosphodiesterase 4D antibody
    see all

Anti-PDE4 antibody 图像

  • ab 14628 detecting 3 isoforms of PDE4A subclasses (PDE4A1, PDE4A5 and PDE4Ax) and two isoforms of PDE4D (PDE4D1, PDE4D3) in rat brain by Western blot.

  • ICC/IF image of ab14628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14628, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PDE4 antibody (ab14628)参考文献

This product has been referenced in:
  • Giralt A  et al. Increased PKA signaling disrupts recognition memory and spatial memory: role in Huntington's disease. Hum Mol Genet : (2011). Read more (PubMed: 21835884) »
  • De Arcangelis V  et al. Equilibrium between adenylyl cyclase and phosphodiesterase patterns adrenergic agonist dose-dependent spatiotemporal cAMP/protein kinase A activities in cardiomyocytes. Mol Pharmacol 78:340-9 (2010). WB ; Human . Read more (PubMed: 20530128) »

See all 6 Publications for this product

Product Wall

I have received a reply from the originator of the product and have attached it below. "Yes, this band was initially characterized as PDE4B4 (before Graemme bolger cloned the PDE4B4) as reported in Biology of Reproduction paper, 2001." I hope t...

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Thank you for your patience. I have contacted the originator of the product for the information you require. Please find the molecular weight band information below. Molecular Wt: PDE4A5 110kDa PDE4Ax 102kDa PDE4D1 68 kDa PDE4D3 95 kDa PD...

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Thank you for your enquiry. This antibody will label most of the PDE4 proteins from PDE4A (PDE4A1,65 kDa; PDE4A5, 106 kDa, PDE4Ax 102 kDa);in PDE4B (generally it labels only PDE4B3 (100 kDa); PDE4D (PDE4D1, D2, D3, D4, D5) provided these proteins are e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"