概述

  • 产品名称Anti-PDE3B抗体
    参阅全部 PDE3B 一抗
  • 描述
    兔多克隆抗体to PDE3B
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Chicken, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1062 and 1112 of human PDE3B. (NP_000913.2)

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab99290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism.
  • 组织特异性Abundant in adipose tissues.
  • 序列相似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5''-cyclic phosphodiesterase B antibody
    • Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
    • AI847709 antibody
    • cAMP Phosphodiesterase, adipocyte, cGMP-inhibited antibody
    • CGI-PDE B antibody
    • CGIP1 antibody
    • CGIPDE B antibody
    • CGIPDE1 antibody
    • cGMP inhibited 3 5 cyclic phosphodiesterase B antibody
    • CGMP inhibited cAMP phophodiesterase antibody
    • cGMP-inhibited 3'' antibody
    • Cyclic GMP inhibited phosphodiesterase B antibody
    • Cyclic GMP-inhibited phosphodiesterase B antibody
    • Cyclic nucleotide phosphodiesterase antibody
    • HcGIP1 antibody
    • HcGIP1, rat, homolog of antibody
    • Pde3b antibody
    • PDE3B_HUMAN antibody
    • Phosphodiesterase 3B cGMP inhibited antibody
    see all

Anti-PDE3B antibody 图像

  • All lanes : Anti-PDE3B antibody (ab99290) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 124 kDa
  • Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody recognising an upstream epitope of PDE3B.
    Lane 2: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99290 at 3 µg/mg lysate for IP.
    Lane 3 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab99290 at 1 µg/ml. Developed using the ECL technique with an exposure time of 10 seconds.

Anti-PDE3B antibody (ab99290)参考文献

ab99290 has not yet been referenced specifically in any publications.

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