RabMAb

Anti-PDCD6抗体[EP3259] (ab109181)

概述

  • 产品名称Anti-PDCD6抗体[EP3259]
    参阅全部 PDCD6 一抗
  • 描述
    兔单克隆抗体[EP3259] to PDCD6
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide against Human PDCD6

  • 阳性对照
    • Jurkat, HeLa and 293T cell lysates, Human hepatoma and Human lung adenocarcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5.
    • 序列相似性Contains 5 EF-hand domains.
    • 细胞定位Nucleus membrane. Endoplasmic reticulum membrane. Nucleus. Endosome. Interaction with RBM22 induces relocalization from the cytoplasm to the nucleus. Translocated from the cytoplasm to the nucleus after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AIP1 antibody
      • ALG 2 antibody
      • ALG-2-interacting protein 1 antibody
      • ALG2 antibody
      • ALIX antibody
      • Apoptosis linked gene 2 antibody
      • Apoptosis linked gene 2 protein antibody
      • Apoptosis-linked gene 2 protein antibody
      • FLJ42309 antibody
      • FLJ46208 antibody
      • Hp95 antibody
      • KIAA1375 antibody
      • MA 3 antibody
      • MA3 antibody
      • MGC111017 antibody
      • MGC119050 antibody
      • MGC9123 antibody
      • PDCD 6 antibody
      • Pdcd6 antibody
      • PDCD6_HUMAN antibody
      • PEF 1B antibody
      • PEF1B antibody
      • Probable calcium binding protein ALG 2 antibody
      • Probable calcium binding protein ALG2 antibody
      • Probable calcium-binding protein ALG-2 antibody
      • Programmed cell death 6 antibody
      • Programmed cell death 6-interacting protein antibody
      • Programmed cell death protein 6 antibody
      • PS 2 antibody
      • PS2 antibody
      see all

    Anti-PDCD6 antibody [EP3259] 图像

    • All lanes : Anti-PDCD6 antibody [EP3259] (ab109181) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 21 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human hepatoma using ab109181 at 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma using ab109181 at 1/100.

    Anti-PDCD6 antibody [EP3259] (ab109181)参考文献

    ab109181 has not yet been referenced specifically in any publications.

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