RabMAb

Anti-PDCD4抗体[EPR3432] (ab79405)

概述

  • 产品名称Anti-PDCD4抗体[EPR3432]
    参阅全部 PDCD4 一抗
  • 描述
    兔单克隆抗体[EPR3432] to PDCD4
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human PDCD4

  • 阳性对照
    • Jurkat and HeLa cell lysates; human breast carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-PDCD4 antibody (Phycoerythrin) [EPR3432] (ab209301)

    Anti-PDCD4 antibody (Alexa Fluor® 405) [EPR3432] (ab210146)

性能

应用

Our Abpromise guarantee covers the use of ab79405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
    • 组织特异性Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
    • 序列相似性Belongs to the PDCD4 family.
      Contains 2 MI domains.
    • 结构域Binds EIF4A1 via both MI domains.
    • 翻译后修饰Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
    • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Death up-regulated gene protein antibody
      • Dug antibody
      • H731 antibody
      • Ma3 antibody
      • MGC33046 antibody
      • MGC33047 antibody
      • Neoplastic transformation inhibitor antibody
      • Neoplastic transformation inhibitor protein antibody
      • Nuclear antigen H731 antibody
      • Nuclear antigen H731 like antibody
      • Nuclear antigen H731 like protein antibody
      • Nuclear antigen H731-like antibody
      • PDCD 4 antibody
      • Pdcd4 antibody
      • PDCD4_HUMAN antibody
      • Programmed cell death 4 antibody
      • programmed cell death 4 (neoplastic transformation inhibitor) antibody
      • Programmed cell death protein 4 antibody
      • Protein 197/15a antibody
      • Protein MA-3 antibody
      • Tis antibody
      • Topoisomerase-inhibitor suppressed protein antibody
      see all

    Anti-PDCD4 antibody [EPR3432] 图像

    • All lanes : Anti-PDCD4 antibody [EPR3432] (ab79405) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
      Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
    • ab79405 at 1/1000 dilution staining PDCD4 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.

    Anti-PDCD4 antibody [EPR3432] (ab79405)参考文献

    ab79405 has not yet been referenced specifically in any publications.

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