概述

  • 产品名称Anti-PDCD4抗体[EPR3431]
    参阅全部 PDCD4 一抗
  • 描述
    兔单克隆抗体[EPR3431] to PDCD4
  • 经测试应用适用于: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminus of Human PDCD4

  • 阳性对照
    • WB: Jurkat, HL60 + RA and HeLa cell lysates; IHC-P: Human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-PDCD4 antibody (Alexa Fluor® 488) [EPR3431] (ab205447)

    Anti-PDCD4 antibody (HRP) [EPR3431] (ab205842)

     

性能

应用

Our Abpromise guarantee covers the use of ab80590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000 - 1/100000. Predicted molecular weight: 52 kDa.
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

靶标

  • 功能Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • 组织特异性Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • 序列相似性Belongs to the PDCD4 family.
    Contains 2 MI domains.
  • 结构域Binds EIF4A1 via both MI domains.
  • 翻译后修饰Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 antibody [EPR3431] 图像

  • All lanes : Anti-PDCD4 antibody [EPR3431] (ab80590) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HL60 + RA lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 ab80590.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-PDCD4 antibody [EPR3431] (ab80590)参考文献

This product has been referenced in:
  • Liu X  et al. MicroRNA-499-5p promotes cellular invasion and tumor metastasis in colorectal cancer by targeting FOXO4 and PDCD4. Carcinogenesis 32:1798-805 (2011). WB ; Human . Read more (PubMed: 21934092) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab80590.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"