概述

  • 产品名称Anti-PDCD4抗体
    参阅全部 PDCD4 一抗
  • 描述
    小鼠多克隆抗体to PDCD4
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human PDCD4 protein (AAH26104.1).

  • 阳性对照
    • Human pancreas lysate, PDCD4 transfected 293T cell lysate, HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab89940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • 组织特异性Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • 序列相似性Belongs to the PDCD4 family.
    Contains 2 MI domains.
  • 结构域Binds EIF4A1 via both MI domains.
  • 翻译后修饰Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 antibody 图像

  • Anti-PDCD4 antibody (ab89940) at 1 µg/ml + human pancreas lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • All lanes : Anti-PDCD4 antibody (ab89940) at 1 µg/ml

    Lane 1 : PDCD4 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab89940, at 10 µg/ml, staining PDCD4 in HeLa cell by Immunofluorescence.

Anti-PDCD4 antibody (ab89940)参考文献

ab89940 has not yet been referenced specifically in any publications.

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