Anti-PD1抗体[RMP1-14] (ab63477)

概述

  • 产品名称
    Anti-PD1抗体[RMP1-14]
    参阅全部 PD1 一抗
  • 描述
    大鼠单克隆抗体[RMP1-14] to PD1
  • 经测试应用
    适用于: IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    PDCD1 transfected BHK cells.

  • 常规说明


    Antibodies produced by clone RMP1-14 have been reported to block the binding of both B7-H1-Ig and B7-DC-Ig fusion proteins to PDCD1 transfected BHK cells.

性能

应用

Our Abpromise guarantee covers the use of ab63477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.

Formaldehyde-based fixatives such as PFA are not recommended. Fix frozen, unfixed tissue sections on the slide with ice-cold acetone for 15-20 minutes.

Flow Cyt 1/10 - 1/25. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Possible cell death inducer, in association with other factors.
  • 疾病相关
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 序列相似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 发展阶段
    Induced at programmed cell death.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

Anti-PD1 antibody [RMP1-14] (ab63477)参考文献

This product has been referenced in:
  • Gray MJ  et al. Phosphatidylserine-targeting antibodies augment the anti-tumorigenic activity of anti-PD-1 therapy by enhancing immune activation and downregulating pro-oncogenic factors induced by T-cell checkpoint inhibition in murine triple-negative breast cancers. Breast Cancer Res 18:50 (2016). Read more (PubMed: 27169467) »
  • Ehlers M  et al. Immunoregulatory natural killer cells suppress autoimmunity by down-regulating antigen-specific CD8+ T cells in mice. Endocrinology 153:4367-79 (2012). Read more (PubMed: 22733969) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (lymphocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

提交于 Jan 05 2017

The amino acid sequence of the antibody itself has not been determined, to our knowledge.

If you need the sequence of the immunogen that generated the antibody, the immunogen and immunization procedure are described in a paper by the laborator...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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