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Syrian Hamster kidney cell line BKH transfected with Mouse PD1 cDNA
It is reported that monoclonal antibody J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.
Our Abpromise guarantee covers the use of ab95790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.25µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.|
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