Anti-PD-L1抗体[MIH6] - Low endotoxin, Azide free (ab80276)

概述

  • 产品名称
    Anti-PD-L1抗体[MIH6] - Low endotoxin, Azide free
    参阅全部 PD-L1 一抗
  • 描述
    大鼠单克隆抗体[MIH6] to PD-L1 - Low endotoxin, Azide free
  • 经测试应用
    适用于: Inhibition Assay, IHC-P, IP, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Mouse PD-L1. Mouse CD274-transfected L5178Y cells

  • 阳性对照
    • Peripheral mouse lymphocytes.

性能

应用

Our Abpromise guarantee covers the use of ab80276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Inhibition Assay Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • 组织特异性
    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位
    Cell membrane and Endomembrane system.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B7 H antibody
    • B7 H1 antibody
    • B7 homolog 1 antibody
    • B7-H1 antibody
    • B7H antibody
    • B7H1 antibody
    • CD 274 antibody
    • CD274 antibody
    • CD274 antigen antibody
    • CD274 molecule antibody
    • MGC142294 antibody
    • MGC142296 antibody
    • OTTHUMP00000021029 antibody
    • PD L1 antibody
    • PD-L1 antibody
    • PD1L1_HUMAN antibody
    • PDCD1 ligand 1 antibody
    • PDCD1L1 antibody
    • PDCD1LG1 antibody
    • PDL 1 antibody
    • PDL1 antibody
    • Programmed cell death 1 ligand 1 antibody
    • Programmed death ligand 1 antibody
    • RGD1566211 antibody
    see all

Anti-PD-L1 antibody [MIH6] - Low endotoxin, Azide free 图像

  • Immunohistochemical analysis of sections from ID8 induced tumours in mice, showing staining of control samples (top row) and samples from mice treated with Trabectedin (bottom row). Samples were fixed in paraformaldehyde and blocked in 5% BSA for 45 minutes at room temperature. They were incubated with ab80726 (diluted 1:100 in PBS) for 16 hours at 4°C to label PD-L1. The secondary antibody used was an Alexa Fluor® 488-conjugated goat anti-rat IgG (H + L) polyclonal (ab150157) at 1/200. The first column shows DAPI staining, the second column shows PD-L1 staining, and the final column shows DAPI and PD-L1 merged. 

  • ab80276 at 10µl/106 cells staining CD274 in Peripheral Mouse Lymphocytes by Flow Cytometry.
  • Ab80276 staining PD-L1 in Mouse lung tissue sections by Immunohistochemistry (IHC-P). Tissue was fixed with formaldehyde and blocked with 2% milk for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer and permeabilized with Triton. Samples were incubated with primary antibody (1/50 in 2% milk in TBS Triton) for 16 hours at 4°C. A biotinylated Anti- rat in Rabbit was used as the secondary antibody.

    See Abreview

  • Ab80276 staining PD-L1 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P). Tissue was fixed with formalin and blocked with 2% BSA for 2 hours at 25°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/50 in 2% BSA) for 20 hours at 4°C. A SignalStain® Boost IHC Detection Reagent-Cell Sign (HRP, rabbit) was used as the secondary antibody.

    See Abreview

Anti-PD-L1 antibody [MIH6] - Low endotoxin, Azide free (ab80276)参考文献

This product has been referenced in:
  • Liu Y  et al. Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1(+)Treg cells. Nat Commun 8:14709 (2017). IF ; Mouse . Read more (PubMed: 28436428) »
  • Gray MJ  et al. Phosphatidylserine-targeting antibodies augment the anti-tumorigenic activity of anti-PD-1 therapy by enhancing immune activation and downregulating pro-oncogenic factors induced by T-cell checkpoint inhibition in murine triple-negative breast cancers. Breast Cancer Res 18:50 (2016). Read more (PubMed: 27169467) »

See all 3 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Specification
Pancreas
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formalin
Username

Abcam user community

Verified customer

提交于 Feb 22 2017

We do not currently have any antibodies that are known to inhibit/neutralize either human PD-L1 or PD1 but ab80276, also known as clone MIH6, may be the best choice, though we do not have data for human reactivity, just mouse. The paper at J Exp Med. 2...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Lung Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton
Specification
Mouse Lung Tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Formaldehyde
Username

Ayman Oweida

Verified customer

提交于 Mar 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Bos taurus Tissue sections (Ovary (corpus luteum) and spleen as positive contr)
Permeabilization
No
Specification
Ovary (corpus luteum) and spleen as positive contr
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Jan 06 2016

Thank you for your enquiry. I can confirm that this product can also be used in an inhibition assay and I have changed the FuncS (Functional assay) application to Inhib (Inhibition assay) to better reflect the product's usage. Also, I was able...

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