Recombinant fragment: DAQCSAKVNA RKRRKEAPGP NGATEEDGVP SKVQRCAVGL RQPAPFSDEI EVDFSKPYVR VTMEEASRGT PCERPVRVYA DGIFDLFHS, corresponding to amino acids 2-91 of Human PCYT1A (NP_005008) with a 26 kDa tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
Use at an assay dependent dilution. Detection limit for recombinant tagged PCYT1A is approximately 3 ng/ml when used as a capture antibody.
功能Controls phosphatidylcholine synthesis.
通路Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
序列相似性Belongs to the cytidylyltransferase family.
翻译后修饰The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
细胞定位Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.