Anti-PCYT1A抗体(ab77305)

概述

  • 产品名称Anti-PCYT1A抗体
    参阅全部 PCYT1A 一抗
  • 描述
    小鼠单克隆抗体to PCYT1A
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Horse, Cow
  • 免疫原

    Recombinant fragment: DAQCSAKVNA RKRRKEAPGP NGATEEDGVP SKVQRCAVGL RQPAPFSDEI EVDFSKPYVR VTMEEASRGT PCERPVRVYA DGIFDLFHS, corresponding to amino acids 2-91 of Human PCYT1A (NP_005008) with a 26 kDa tag.

  • 阳性对照
    • PC-12 and K-562 cell lysates. Recombinant tagged human PCYT1A fragment (immunogen).

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab77305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged PCYT1A is approximately 3 ng/ml when used as a capture antibody.

靶标

  • 功能Controls phosphatidylcholine synthesis.
  • 通路Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
  • 序列相似性Belongs to the cytidylyltransferase family.
  • 翻译后修饰The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
  • 细胞定位Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCT A antibody
    • CCT alpha antibody
    • CCT-alpha antibody
    • Choline phosphate cytidylyltransferase A antibody
    • Choline-phosphate cytidylyltransferase A antibody
    • CT A antibody
    • CT antibody
    • CTP:phosphocholine cytidylyltransferase A antibody
    • CTPCT antibody
    • PCY1A_HUMAN antibody
    • PCYT1 antibody
    • Pcyt1a antibody
    • Phosphate cytidylyltransferase 1 choline alpha antibody
    • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
    • Phosphorylcholine transferase A antibody
    see all

Anti-PCYT1A antibody 图像

  • Anti-PCYT1A antibody (ab77305) at 5 µg/ml + recombinant tagged human PYCT1A fragment (the immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 42 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-PCYT1A antibody (ab77305) at 5 µg/ml + PC-12 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • Anti-PCYT1A antibody (ab77305) at 5 µg/ml + K-562 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

Anti-PCYT1A antibody (ab77305)参考文献

ab77305 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Hindlimb muscle)
Loading amount 40 µg
Specification Hindlimb muscle
Gel Running Conditions Reduced Denaturing (gradient 4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. David Russ

Verified customer

提交于 Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"