概述

  • 产品名称
  • 描述
    兔多克隆抗体to PCYOX1
  • 经测试应用
    适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    A region within synthetic peptide: PSTDGTYVWK IFSQETLTKA QILKLFLSYD YAVKKPWLAY PHYKPPEKCP, corresponding to C terminal amino acids 397-446 of Human PCYOX1

  • 阳性对照
    • Jurkat cell lysate and human liver tissue

性能

应用

Our Abpromise guarantee covers the use of ab49347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the prenylcysteine oxidase family.
  • 翻译后修饰
    The protein is glycosylated at one or more potential N-glycosylation sites.
  • 细胞定位
    Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 1.8.3.5 antibody
    • KIAA0908 antibody
    • PCL1 antibody
    • PCYOX_HUMAN antibody
    • PCYOX1 antibody
    • Prenylcysteine lyase antibody
    • Prenylcysteine oxidase 1 antibody
    • Prenylcysteine oxidase precursor antibody
    see all

图片

  • Anti-PCYOX1 antibody (ab49347) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 -1/100,000

    Predicted band size : 57 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • This image shows human liver tissue stained with ab49347 at 4µg/ml. Positive label (arrows) are hematopoietic cells. Magnification X400.

文献

ab49347 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab49347.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册