概述

  • 产品名称Anti-PCYOX1抗体
    参阅全部 PCYOX1 一抗
  • 描述
    兔多克隆抗体to PCYOX1
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    A region within synthetic peptide: PSTDGTYVWK IFSQETLTKA QILKLFLSYD YAVKKPWLAY PHYKPPEKCP, corresponding to C terminal amino acids 397-446 of Human PCYOX1

  • 阳性对照
    • Jurkat cell lysate and human liver tissue

性能

应用

Our Abpromise guarantee covers the use of ab49347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the prenylcysteine oxidase family.
  • 翻译后修饰The protein is glycosylated at one or more potential N-glycosylation sites.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 1.8.3.5 antibody
    • KIAA0908 antibody
    • PCL1 antibody
    • PCYOX_HUMAN antibody
    • PCYOX1 antibody
    • Prenylcysteine lyase antibody
    • Prenylcysteine oxidase 1 antibody
    • Prenylcysteine oxidase precursor antibody
    see all

Anti-PCYOX1 antibody 图像

  • Anti-PCYOX1 antibody (ab49347) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 -1/100,000

    Predicted band size : 57 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • This image shows human liver tissue stained with ab49347 at 4µg/ml. Positive label (arrows) are hematopoietic cells. Magnification X400.

Anti-PCYOX1 antibody (ab49347)参考文献

ab49347 has not yet been referenced specifically in any publications.

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