使用敲除细胞株进行验证

Anti-PCTAIRE1抗体(ab55553)

概述

  • 产品名称Anti-PCTAIRE1抗体
    参阅全部 PCTAIRE1 一抗
  • 描述
    小鼠单克隆抗体to PCTAIRE1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MDRMKKIKRQ LSMTLRGGRG IDKTNGAPEQ IGLDESGGGG GSDPGEAPTR AAPGELRSAR GPLSSAPEIV HEDLKMGSDG , corresponding to amino acids 1-80 of Human PCTAIRE1

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May play a role in signal transduction cascades in terminally differentiated cells.
    • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cdk16 antibody
      • CDK16_HUMAN antibody
      • Cell division protein kinase 16 antibody
      • Cyclin-dependent kinase 16 antibody
      • PCTAIRE antibody
      • PCTAIRE protein kinase 1 antibody
      • PCTAIRE-motif protein kinase 1 antibody
      • PCTAIRE1 antibody
      • PCTGAIRE antibody
      • PCTK1 antibody
      • Serine/threonine-protein kinase PCTAIRE-1 antibody
      see all

    Anti-PCTAIRE1 antibody 图像



    • Predicted band size : 56 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: PCTAIRE1 knockout HAP1 cell lysate (40 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab55553 observed at 63 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab55553 was shown to recognize PCTAIRE1  when PCTAIRE1  knockout samples were used, along with additional cross-reactive bands. Wild-type and PCTAIRE1  knockout samples were subjected to SDS-PAGE. Ab55553 and ab181602 (loading control to GAPDH) were diluted at 1 µg/ml  and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.



    • Predicted band size : 56 kDa
      PCTAIRE1 antibody (ab55553) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    Anti-PCTAIRE1 antibody (ab55553)参考文献

    ab55553 has not yet been referenced specifically in any publications.

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