概述

  • 产品名称Anti-PCSK9抗体
    参阅全部 PCSK9 一抗
  • 描述
    兔多克隆抗体to PCSK9
  • 特异性Detects both the zymogen and active forms of PC9. Smaller cleavage products are also detected.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human PCSK9 (C terminal). From the propeptide cleavage site. Based on the catalytic domain.

性能

应用

Our Abpromise guarantee covers the use of ab52754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Predicted molecular weight: 74 kDa. 1/1000 when using colorimetric substrates and 1/5000 for chemiluminescent substrates. (pre-pro-peptide runs at 78-80 kDa, the mature form at 58 kDa)

靶标

  • 功能May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • 组织特异性Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • 疾病相关Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • 序列相似性Belongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • 翻译后修饰The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Convertase subtilisin/kexin type 9 preproprotein antibody
    • FH3 antibody
    • HCHOLA3 antibody
    • Hypercholesterolemia autosomal dominant 3 antibody
    • LDLCQ1 antibody
    • NARC 1 antibody
    • NARC-1 antibody
    • NARC1 antibody
    • Neural apoptosis regulated convertase 1 antibody
    • Neural apoptosis-regulated convertase 1 antibody
    • PC 9 antibody
    • PC9 antibody
    • PCSK 9 antibody
    • PCSK9 antibody
    • PCSK9_HUMAN antibody
    • Proprotein convertase 9 antibody
    • Proprotein convertase PC9 antibody
    • Proprotein convertase subtilisin/kexin type 9 antibody
    • PSEC0052 antibody
    • Subtilisin/kexin like protease PC9 antibody
    • Subtilisin/kexin-like protease PC9 antibody
    see all

Anti-PCSK9 antibody 图像

  • ICC/IF image of ab52754 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52754, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PCSK9 antibody (ab52754)参考文献

ab52754 has not yet been referenced specifically in any publications.

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