Anti-PCNA抗体[PC10 (a.k.a. 3F81)] (Phycoerythrin) (ab93576)

概述

  • 产品名称
    Anti-PCNA抗体[PC10 (a.k.a. 3F81)] (Phycoerythrin)
    参阅全部 PCNA 一抗
  • 描述
    小鼠单克隆抗体[PC10 (a.k.a. 3F81)] to PCNA (Phycoerythrin)
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用
    适用于: IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Protein A-Rat PCNA fusion obtained from pC2T.

  • 阳性对照
    • Alcohol fixed MOLT-4 cells

性能

应用

Our Abpromise guarantee covers the use of ab93576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
Flow Cyt
  • 应用说明
    Flow Cyt: Use 20 µl (or 0.125 µg) for 105-8 cells in a 100 µl volume per test.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
    • 序列相似性
      Belongs to the PCNA family.
    • 翻译后修饰
      Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
      Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
    • 细胞定位
      Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATLD2 antibody
      • cb16 antibody
      • Cyclin antibody
      • DNA polymerase delta auxiliary protein antibody
      • etID36690.10 antibody
      • fa28e03 antibody
      • fb36g03 antibody
      • HGCN8729 antibody
      • MGC8367 antibody
      • Mutagen-sensitive 209 protein antibody
      • OTTHUMP00000030189 antibody
      • OTTHUMP00000030190 antibody
      • PCNA antibody
      • Pcna/cyclin antibody
      • PCNA_HUMAN antibody
      • PCNAR antibody
      • Polymerase delta accessory protein antibody
      • Proliferating cell nuclear antigen antibody
      • wu:fa28e03 antibody
      • wu:fb36g03 antibody
      see all

    图片

    • Intracellular staining of Molt-4 cells with FITC conjugated anti-Human PCNA (PC10) (left), and ab93576 (right). Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

    文献

    ab93576 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 28 2013

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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