Anti-PCNA抗体[5E6/2] (ab70472)
Key features and details
- Mouse monoclonal [5E6/2] to PCNA
- Suitable for: IP, ELISA, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
概述
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产品名称
Anti-PCNA抗体[5E6/2]
参阅全部 PCNA 一抗 -
描述
小鼠单克隆抗体[5E6/2] to PCNA -
宿主
Mouse -
经测试应用
适用于: IP, ELISA, WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein corresponding to Saccharomyces cerevisiae PCNA. Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.
Database link: P15873 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
5E6/2 -
骨髓瘤
Sp2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70472于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
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说明 |
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IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
靶标
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功能
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
序列相似性
Belongs to the PCNA family. -
翻译后修饰
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
细胞定位
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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数据库链接
- Entrez Gene: 852385 Saccharomyces cerevisiae
- SwissProt: P15873 Saccharomyces cerevisiae
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别名
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab70472 被引用在 15 文献中.
- Brown RE et al. The RNA export and RNA decay complexes THO and TRAMP prevent transcription-replication conflicts, DNA breaks, and CAG repeat contractions. PLoS Biol 20:e3001940 (2022). PubMed: 36574440
- Reyes GX et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair. Curr Biol 31:1268-1276.e6 (2021). PubMed: 33417883
- Guilliam TA & Yeeles JTP Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart. Nat Struct Mol Biol 27:450-460 (2020). PubMed: 32341533
- Kulkarni DS et al. PCNA activates the MutL? endonuclease to promote meiotic crossing over. Nature 586:623-627 (2020). PubMed: 32814343
- Paul Solomon Devakumar LJ et al. Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex. Nucleic Acids Res 47:6826-6841 (2019). PubMed: 31114918