- 数据表
- 具体的参考文献 (5)
- 实验方案
概述
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产品名称Anti-PCNA抗体[5E6/2]
参阅全部 PCNA 一抗 -
描述小鼠单克隆抗体[5E6/2] to PCNA
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经测试应用适用于: WB, IP, ELISAmore details
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种属反应性与反应: Saccharomyces cerevisiae
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免疫原
Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: None
Constituents: PBS -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号5E6/2
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骨髓瘤Sp2
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同种型IgG1
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab70472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. Predicted molecular weight: 29 kDa. | |
| IP | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. |
靶标
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功能This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
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序列相似性Belongs to the PCNA family.
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翻译后修饰Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
细胞定位Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
- Information by UniProt
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数据库链接
- SwissProt: P15873 Saccharomyces cerevisiae
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别名
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Anti-PCNA antibody [5E6/2] (ab70472)参考文献
This product has been referenced in:
- Johnson C et al. PCNA Retention on DNA into G2/M Phase Causes Genome Instability in Cells Lacking Elg1. Cell Rep 16:684-95 (2016). Read more (PubMed: 27373149) »
- Kubota T et al. Replication-Coupled PCNA Unloading by the Elg1 Complex Occurs Genome-wide and Requires Okazaki Fragment Ligation. Cell Rep 12:774-87 (2015). Read more (PubMed: 26212319) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"