概述

  • 产品名称
  • 描述
    兔多克隆抗体to PCMTD1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: FVGNQLIPQP LDSEEDEKME EDNKEEEEKD HNEAMKPEEP PQNLLREKIM KLPLPE, corresponding to internal sequence amino acids 290-345 of Human PCMTD1.

  • 阳性对照
    • Human duodenum tissue, RT-4 cell line lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 序列相似性
    Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PCMD1_HUMAN antibody
    • PCMTD1 antibody
    • Protein L isoaspartate (D aspartate) O methyltransferase domain containing 1 antibody
    • Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 antibody

图片

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus. Recommended concentration of ab121858 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121858, at 1/600 dilution, staining PCMTD1 in glandular cells of paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-PCMTD1 antibody (ab121858) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

文献

ab121858 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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