Anti-PCMT1抗体(ab55510)

概述

  • 产品名称Anti-PCMT1抗体
    参阅全部 PCMT1 一抗
  • 描述
    小鼠单克隆抗体to PCMT1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-229 of Human PCMT1

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性PCMT1 (Protein-L-isoaspartate (D-aspartate) O-methyltransferase) plays a role in the repair and/or degradation of damaged proteins by initiating the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form. It catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • L isoaspartyl protein carboxyl methyltransferase antibody
      • L isoaspartyl/D aspartyl methyltransferase antibody
      • PCMT 1 antibody
      • PIMT antibody
      • Protein beta aspartate methyltransferase antibody
      • Protein L isoaspartate (D aspartate) O methyltransferase antibody
      • Protein L isoaspartyl/D aspartyl methyltransferase antibody
      see all

    Anti-PCMT1 antibody 图像



    • Predicted band size : 25 kDa
    • All lanes : Anti-PCMT1 antibody (ab55510) at 1/1000 dilution

      Lane 1 : Whole cell lysates prepared from human 293T cells
      Lane 2 : Whole cell lysate prepared from human Hela cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa Fluor® 680 conjugated goat polyclonal to mouse Ig at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 25 kDa


      Exposure time : 2 minutes

      This image is courtesy of an anonymous Abreview

    Anti-PCMT1 antibody (ab55510)参考文献

    ab55510 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T (lane 1) and Hela (lane 2))
    Loading amount 20 µg
    Specification 293T (lane 1) and Hela (lane 2)
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Nov 17 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"